Relationship of microsatellite instability with cervical carcinoma

Abstract

Objective To investigate the relationship of cervical carcinoma with microsatellite instability,so as to provide a new pathway for investigating the risks of cervical carcinoma.Methods PCR and electrophoresis method were used to conduct genotype analysis on D1S1627 and D22S1045 in the peripheral blood of 120 cervical carcinoma patients and 102 healthy women living in Changzhou area.The susceptible and(or) resistant factors of cervical carcinoma were evaluated based on the differences in allele distribution of D1S1627 and D22S1045.Results There were significant differences in locus D22S1045(P=0.05)between cervical carcinoma group and healthy group,not on locus D1S1627(P=0.995).Univariate analysis showed significant differences at allele 14 and 17 on locus D22S1045 between the two groups(P=0.026,P=0.014),with the OR values being 3.837(95%CI:1.078-13.658) and 0.520(95%CI:0.306-0.882),respectively.Multivariate logistic regression analysis results showed that parity,smoking and allele 14 of D22S1045 were the risk factors of cervical carcinoma,with the OR value being 2.063(95%CI:1.070-3.975),1.958(95%CI:1.002-3.826) and 2.766(95%CI:1.039-7.360),respectively.Conclusion Locus D22S1045 might be associated with cervical carcinoma,with allele 14 being the susceptible factor and cellele 17 being the resistant factor.