Expression and clinical significance of high mobility group box-1 and Hepsin in cervical carcinoma tissues

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Objective To explore expression and clinical significance of high mobility group box (HMGB)-1 and Hepsin in cervical carcinoma. Methods A total of 70 cases of cervical carcinoma patients from May 2008 to June 2010 in Xuzhou Central Hospital were selected as study subjects. Seventy cases of surgical ablated cervical carcinoma tissues and 70 cases of para-cancer tissues of 2 cm near to cancer, were included in cervical carcinoma group and para-cancer group; and 20 cases of benign lesion cervical tissues which were obtained from cervical tumor enucleation or adnexectomy because of benign cervical lesions in the same period in same hospital were selected as the control groups. The expression of HMGB-1 and Hepsin proteins in these 3 groups were detected by immunohistochemistry streptavidin-perosidase (SP) method.The differences of expression levels of HMGB-1 protein and Hepsin protein in 3 groups and the differences of positive expression rates of HMGB-1 and Hepsin proteins in cervical carcinoma tissue in different clinical factors were compared statistically.Analyzed the correlation between HMGB-1 protein and Hepsin protein expression level in cervical carcinoma tissue.And the survival analysis of cervical carcinoma patients with different expression intensities of 2 kinds of proteins were conducted. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Xuzhou Central Hospital.Informed consent was obtained from each participant. Results ①The positive expression rates of HMGB-1 and Hepsin proteins in cervical carcinoma group were higher than those of para-cancer group and control group, respectively, also the positive expression rates of HMGB-1 and Hepsin proteins in para-cancer group were higher than those of control group, and the differences were statistically significant(P<0.05). ②Immunohistochemical SP staining results showed that the HMGB-1 and Hepsin proteins were negative expression in benign lesion cervical tissues, pale yellow or yellow particles of positive expression(+ + ) in cancer adjacent tissues, brown particles of positive expression(+ + + ) in cervical carcinoma tissues and mainly located in the cytoplasm.③In 70 cases cervical carcinoma tissues, the positive expression rates of HMGB-1 protein of patients with tumor invasion depth of T3+ T4 and lymph node metastasis were higher than that of patients with tumor invasion depth of T1+ T2 and without lymph node metastasis, and the differences were statistically significant(P<0.05); the positive expression rates of Hepsin protein of patients with low differentiation tumors, tumor invasion depth of T3+ T4, lymph node metastasis, and tumor, node, metastasis(TNM) stages of Ⅲ+ Ⅳ were higher than that of patients with high or middle differentiation tumors, tumor invasion depth of T1+ T2, without lymph node metastasis and tumor TNM stages of Ⅰ+ Ⅱ, and the differences were statistically significant(P<0.05). ④There was a positive correlation between HMGB-1 protein and Hepsin protein expression level in cervical carcinoma tissue (r=15.27, P=0.01). ⑤The risk factors of 5-year survival outcomes in cervical carcinoma patients including HMGB-1 protein (+ + + ) expression(HR=11.637, 95%CI: 4.351-38.213, P<0.05) and Hepsin protein (+ + + ) expression(HR=10.142, 95%CI: 4.285-33.275, P<0.05). Conclusions The over expression level of HMGB-1 and Hepsin proteins were related to the increased invasion of cervical carcinoma.The expression level of these 2 kinds of protein might act as indicators for the prognostic evaluation of cervical carcinoma patients. Key words: Uterine cervical neoplasms; HMGB1 protein; Hepsin; Neoplasm invasiveness; Female