Abstract
Objective To observe the change of bladder compliance and bladder interstitial cells of Cajal (ICCs) post-suprasacral spinal cord transection at different time points in rats and the effect of Imatinib mesylate on the complicance of neurogenic bladder caused by long-term suprasacral spinal cord transaction in rats. Methods One hundred and two female Wistar rats were randomly divided into 6 groups: group A (sham group of 6 weeks); group B (suprasacral spinal cord transaction group of 6 weeks); group C and group E (sham group of 12 weeks); group D and group F (suprasacral spinal cord transaction group of 12 weeks). The rats in groups A to D were used to study the change of bladder compliance and bladder ICCs post-suprasacral spinal cord transection at different time points. The rats in group E and group F were used to study the effect of Imatinib mesylate on the complicance of neurogenic bladder caused by long-term suprasacral spinal cord transaction. Spinal cord transection at T10 level was built in spinal cord transaction groups, while group A, group C and group F underwent sham operaion. Urodynamics was detected at 6th or 12th week after surgery. We observed the changes of ICCs in the bladder wall by immunohistochemistry. Urodynamics was also detected in group E and group F post-surgery 12 weeks before and after intravenous administration of Imatinib mesylate (5 μmol and 20 μmol respectively). Results The bladder compliance was significantly increased [(0.146±0.107) ml/cmH2O vs. (0.066±0.019) ml/cmH2O, P=0.017] and the number of ICCs was significantly decreased [(3.180±1.976) vs. (4.920±1.564), P=0.014] in group B as compared with group A. The bladder compliance was significantly lower [(0.046±0.023) ml/cmH2O vs. (0.063±0.013) ml/cmH2O] and the number of ICCs was significantly higher [(7.710±2.024) vs. (4.750±1.138), P=0.000] in group D than in group C. The bladder compliance in group E was increased by intravenous administration of 20 μmol Imatinib mesylate [from (0.070±0.029) ml/cmH2O to (0.096±0.036) ml/cmH2O]. It increased the bladder compliance of group F in a dose-dependent manner. The bladder compliance in group F was increased by 5 μmol and 20 μmol Imatinib mesylate from (0.047±0.021) ml/cmH2O to (0.065±0.019) ml/cmH2O and (0.077±0.019) ml/cmH2O respectively, P=0.000. Conclusion The bladder compliance was increased first, then decreased and the number of ICCs changed adversely in the T10 spinal cord transection rats. The bladder compliance of the rats at 12th week post-suprasacral spinal cord transection could be improved by inhibiting the ICCs function. Not only the fibrosis, but also the number of ICCs of the bladder wall increased might be the pathological basis of the reduced bladder compliance in suprasacral spinal cord transaction rats. Key words: Spinal cord injury; Neurogenic bladder; Bladder compliance; Interstitial cells of Cajal; Imatinib mesylate
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call