Relationship between stationary and mobile phase composition and its influence on retention factors of aromatic hydrocarbons in reversed-phase high-performance liquid chromatography

Abstract

The effect of the composition of the mobile phase on the composition of the stationary phase and its influence on the retention factor, k′, of polynuclear hydrocarbons has been determined. The decreasing relationship between log k′ and the concentration of modifier (methanol) in mobile and stationary phases has been proved. It has been determined that the solubility of examined aromatic hydrocarbons in stationary phase is a reason for changes in the k′. This fact can be used in order to improve the effectiveness of separation of some mixtures.