Relationship between soil respiration and soil moisture

Abstract

Microbial activity is affected by changes in the availability of soil moisture. We examined the relationship between microbial activity and water potential in a silt loam soil during four successive drying and rewetting cycles. Microbial activity was inferred from the rate of CO 2 accumulating in a sealed flask containing the soil sample and the CO 2 respired was measured using gas chromatography. Thermocouple hygrometry was used to monitor the water potential by burying a thermocouple in the soil sample in the flask. Initial treatment by drying on pressure plates brought samples of the test soil to six different water potentials in the range -0.005 to -1.5MPa. Water potential and soil respiration were simultaneously measured while these six soil samples slowly dried by evaporation and were remoistened four times. The results were consistent with a log-linear relationship between water potential and microbial activity as long as activity was not limited by substrate availability. This relationship appeared to hold for the range of water potentials from −0.01 to −8.5 MPa. Even at −0.01 MPa (wet soil) a decrease in water potential from −0.01 to −0.02 MPa caused a 10% decrease in microbial activity. Rewetting the soil caused a large and rapid increase in the respiration rate. There was up to a 40-fold increase in microbial activity for a short period when the change in water potential following rewetting was greater than 5 MPa. Differences in microbial activity between the wetter and drier soil treatments following rewetting to the original water potentials are discussed in terms of the availability of energy substrate.