Relationship between Golgi protein 73 and outcome of liver disease after hepatitis B virus infection

Abstract

Objective To investigate the relationship between serum N-glycosylated Golgi protein 73 (GP73) expression and outcome of liver disease after HBV infection.Methods A total of 354 serum sample were collected from patients with liver diseases (81 primary hepatic cancer,123 severe hepatitis,57 chronic hepatitis B,and 93 hepatitis B associated liver cirrhosis) in the Six People's Hospital of Shaoxing during October 2010 and May 2013.ELISA was used to detect serurm GP73.Thirty healthy people were enrolled as controls.Results The median serum levels of GP73 in primary hepatic cancer group,severe hepatitis group,liver cirrhosis group,chronic hepatitis B group and healthy controls were 345.2,301.7,262.0,123.5,and 58.6 ng/mL,respectively,with significant differences between every two groups (u=891.0,958.0,1029.5 and 419.0,P＜0.05 or ＜0.01).The median serum level of GP73 in primary hepatic cancer patients with viral load ≥ 3 lg copies/mL (52 cases) was higher than that of primary cancer patients with viral load ＜3 lg copies/mL (29 cases),with a significant difference (328.3 ng/mL vs 284.3 ng/mL,u=533.5,P＜0.01).Serum GP73 expression was negatively related to cholinesterase and albumin (r=-0.57 and-0.59,P＜0.05).Conclusions Serum GP73 expression increases as the liver disease becomes severe and viral load rises.It shows a negative correlation of GP73 with cholinesterase and albumin. Key words: Hepatitis B virus; Golgi Protein 73; Liver cirrhosis; Primary hepatic cancer