Relationship between gene mutations and clinicopathological features in nonampullary duodenal epithelial tumors.

Abstract

Molecular features of nonampullary duodenal epithelial tumors (NADETs) remain unclear. The aim of this study is to determine the association between the genetic features and clinicopathological findings of NADETs. In total, 75 NADETs were enrolled in this study, and was performed targeted DNA sequencing of the GNAS, KRAS, TP53, and APC genes. Histological grade was classified as category 3 or category 4/5 according to the Vienna classification, and the immunophenotype was categorized as the gastric phenotype (G type), gastrointestinal phenotype (GI type), or the intestinal phenotype (I type). The prevalence of GNAS and KRAS mutations was significantly higher in the G type than in the GI/I type (GNAS, P=0.027; KRAS, P=0.005). In contrast, the frequency of TP53 mutations was significantly higher in the GI/I type than in the G type (P=0.049). Notably, APC mutations, excluding c.4479 G>A which was synonymous mutation, were more frequently identified in category 4/5 tumors than in category 3 tumors (50% vs. 24.5%; P=0.039). G-type NADETs harbored frequent GNAS and KRAS mutations, whereas TP53 mutations are common in NADETs with intestinal features. APC mutations were significantly associated with high-grade neoplasia and invasive carcinoma.