Relationship between ApoE gene polymorphism and Lp-PLA2 in patients with acute cerebral infarction

Abstract

Objective To investigate the distribution of ApoE gene polymorphism in patients with acute cerebral infarction (ACI) and to explore the its correlation with the expression of lipoprotein-associated phospholipase A2 (Lp-PLA2). Methods A case-control study was conducted in 112 ACI inpatients that visited the Department of Neurology, the Third People′s Hospital of Liaocheng from July 2015 to June 2017 and 59 age-matched healthy subjects were included as control. Nucleic acid probe hybridization chips were used to detect the genotypes of ApoE and serum Lp-PLA2 was measured by the up-converting luminescence technique. Results The allele frequencies of ApoE E2, E3 and E4 in the control group were 21.19%, 73.73% and 5.08%, respectively. The genotype frequency of ApoE conforms to the balance of Hardy-Weinberg. The frequency of E2 allele in ACI group (6.25%) was significantly lower than that in control group (21.19%) (P 0.05), while there were significant differences among the other groups (P<0.01). The AUC value of serum Lp-PLA2 was 0.790 (95%CI 71.9-86.1). The critical point of clinical diagnosis is 123.01ng/ml,the sensitivity was 67.8%, the specificity was 69.6%, and the expression level of Lp-PLA2 was significant (P<0.01) for judging the occurrence of ACI. Logistic regression analysis showed that Lp-PLA2 and ApoE E4 alleles were both risk factors for the diagnosis and treatment of ACI. Conclusion The ApoE E4 allele is a risk factor for acute cerebral infarction and Lp-PLA2 expression is closely related with ACI patients. The detection of ApoE genotype and serum Lp-PLA2 is of certain clinical significance in the diagnosis of ACI. Key words: Acute cerebral infarction; Apolipoprotein E; Gene polymorphism; Allele; Lipoprotein-associated phospholipase A2