Relations of Ruminal Fermentation Parameters and Microbial Matters to Odd- and Branched-Chain Fatty Acids in Rumen Fluid of Dairy Cows at Different Milk Stages.

Keyuan Liu,Guangyu Li,Yang Li,Hangshu Xin,Guobin Luo,Yonggen Zhang

doi:10.3390/ani9121019

Abstract

Simple SummaryThe objective of this study was to determine the relationships between milk odd- and branched-chain fatty acids (OBCFAs) and ruminal fermentation parameters, microbial populations, and base contents. Significant relationships existed between the concentrations of C11:0, iso-C15:0, anteiso-C15:0, C15:0, and anteiso-C17:0 in rumen and milk. The total OBCFA content in milk was positively related to the acetate molar proportion but negatively correlated with isoacid levels. The adenine/N ratio was negatively related to milk OBCFA content but positively associated with the iso-C15:0/iso-C17:0 ratio.The purpose of this research was to evaluate whether relationships exist between odd- and branched-chain fatty acids (OBCFAs) originating from milk fat and the corresponding data of ruminal fermentation parameters, microbial populations, and base contents that were used to mark microbial protein in rumen. Nine lactating Holstein dairy cows with similar body weights and parity were selected in this study, and the samples of rumen and milk were collected at the early, middle, and late stages, respectively. The rumen and milk samples were collected over three consecutive days from each cow, and the ruminal and milk OBCFA profiles, ruminal fermentation parameters, bacterial populations, and base contents were measured. The results showed that the concentrations of OBCFAs, with the exception of C11:0 and C15:0, were significantly different between milk and rumen (p < 0.05). The concentrations of anteiso-fatty acids in milk were higher than those in rumen, and the contents of linear odd-chain fatty acids were higher than those of branched-chain fatty acids in both milk and rumen. Significant relationships that existed between the concentrations of C11:0, iso-C15:0, anteiso-C15:0, C15:0, and anteiso-C17:0 in rumen and milk (p < 0.05). The total OBCFA content in milk was positively related to the acetate molar proportion but negatively correlated with isoacid contents (p < 0.05). The populations of Ruminococcus albus, R. flavefacients, and Eubacterium ruminantium were significantly related to milk C13:0 contents (p < 0.05). The adenine/N ratio was negatively related to milk OBCFA content (p < 0.05) but positively associated with the iso-C15:0/iso-C17:0 ratio (p < 0.05). Milk OBCFAs were significantly correlated with ruminal fermentation parameters, ruminal bacterial populations, and base contents. Milk OBCFAs had the potential to predict microbial nitrogen flow, and the prediction equations for ruminal microbial nitrogen flow were established for OBCFAs in dairy milk.

Highlights

There is growing awareness that milk fat content can respond to physiological and metabolic health situations [1,2]
Odd- and branched-chain fatty acids (OBCFAs) of ruminant milk generally originate from rumen bacteria [7]
The total OBCFA and odd anteiso-chain fatty acids contents contained in milk were higher than those in rumen, and the linear odd-chain fatty acids were more abundant both in milk and rumen (Figure 1)

Summary

Introduction

There is growing awareness that milk fat content can respond to physiological and metabolic health situations [1,2]. The milk fatty acid profile is a dynamic pattern influenced by many factors, such as lactational stage, season, and dietary composition [3,4,5]. The microbial processes of the rumen confer the ability to convert feeds into available nutrients for the ruminant animal [6]. Odd- and branched-chain fatty acids (OBCFAs) of ruminant milk generally originate from rumen bacteria [7]. Some studies have discussed the potential of OBCFAs as markers of rumen fermentation and ruminal bacteria [9,10,11,12]. Lactating dairy cow digestion is strongly determined by the microbial population in the rumen

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