Regulatory Role of Zinc on the Biokinetics and Biodistribution of 65Zn During the Initiation of Experimentally Induced Colon Cancer

Abstract

The present study was conducted to evaluate the kinetics of zinc utilization during the formation of colon carcinoma in an animal model of colon carcinogenesis. The rats were segregated into 4 groups: untreated control, 1,2 dimethylhydrazine (DMH) treated, zinc treated, and DMH+zinc treated. Colon carcinogenesis was initiated through weekly subcutaneous injections of DMH (30 mg/kg body weight) for 8 wk. Zinc (in the form of zinc sulphate) was supplemented at a dose level of 227mg/L in drinking water, ad libitum for the entire duration of study. Whole body 65Zn kinetics followed two-compartment kinetics, with Tb1 representing the initial fast component of the biological half-life and Tb2, the slower component. The Tb1 component showed a significant elevation while the Tb2 component was significantly diminished in DMH-treated rats, which, however, got normalized following zinc supplementation. The biodistribution and subcellular distribution of 65Zn was significantly affected in DMH-treated rats when compared to normal control rats. However, zinc significantly reversed the altered 65Zn uptake in different organs and various fractions of colon. The present study for the first time demonstrated a faster mobilization of zinc during initiation of experimentally induced colon carcinoma and provides a physiological basis for the role of zinc in colon tumorigenesis.