Regulation Studies of a Cuticle Protein Underlying Genomic Analysis

Ali Ms

doi:10.16966/2471-4968.104

Abstract

Regulation Studies of a Cuticle Protein Underlying Genomic Analysis

Highlights

Insect cuticle is a multi-layered structure with three functional regions: epicuticle, procuticle, and endocuticle, which differs in protein composition, structural features and physiological functions [1].The character of insect cuticle is determined by the construction of cuticular protein, and the various cuticle types show pronounced differences in mechanical properties and these differences are related to the properties of the individual proteins [2]
The promoter region of the different cuticular protein genes was bound and activated by EcR and different ecdysoneresponsive transcription factors, βFTZ-F1, E74A, BR-C Z2, and BR-C Z4, resulting in their different expression patterns. These studies succeeded by using genomic information and a transient reporter assay by using wing discs
We found a cuticular protein gene, CPR95, which transcripts increased rapidly after the W3 early (W3E) stage and peaked at W3 late (W3L), rapidly decreased

Summary

Introduction

Insect cuticle is a multi-layered structure with three functional regions: epicuticle, procuticle, and endocuticle, which differs in protein composition, structural features and physiological functions [1].The character of insect cuticle is determined by the construction of cuticular protein, and the various cuticle types show pronounced differences in mechanical properties and these differences are related to the properties of the individual proteins [2]. The character of insect cuticle differs in species and stages, and the difference is brought about by the combination of cuticular proteins. Ecdysone-responsive expression of cuticular proteins has been reported [3,4,5,6,7,8,9,10,11,12] Most of their expressions were induced by an ecdysteroid pulse; the expression required the existence and removal of 20E [9,11,13]. Cuticular protein genes, which have different developmental profiles, were induced by different types of ecdysone signal, and these things were described in a recent review [14]

Methods

Results

Conclusion