Regulation ofin vitroshoot multiplication inSyringa, AlnusandMalusby different carbon sources

Abstract

SummaryThe influence of different carbon sources on shoot multiplication was studied in the apple rootstock M.9. (Malus pumila Mill.), Syringa chinensis Willd. cv. Saugeana and Alnus glutinosa (L.) Gaertn. Both nodal and shoot tip explants were used. The sugars sucrose, glucose, fructose and the sugar alcohols mannitol and sorbitol at 88 and 175 mM were investigated. The carbon source influenced the percentage explants forming shoots, number of shoots per explant, number of separable nodes per shoot as well as shoot length. In Syringa, the carbohydrates influenced mainly the survival percentage of the explants, with the best carbon source being mannitol at both concentrations and sucrose at 88 mM. Overall the most effective carbon source in Alnus was glucose at 88 mM. Malus explants grew best on sorbitol, and fructose at 175 mM. The capability to metabolize a certain type of carbon source was reflected by the sugars and sugar alcohols reported in the sieve-tube exudate within a given genus (Zimmermann and...