Abstract
In this present study, the effect of various carbon sources such as sucrose, glucose, fructose and maltose was investigated on in vitro shoot regeneration of Solanum viarum using axillary bud explants. The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon source used. Among the different concentrations (1%-6%) of carbohydrates studied, the maximum number of shoots (22.6 ± 0.50) and shoot length (5.92 ± 0.13 cm) was obtained on MS medium supplemented with 4% (w/v) fructose. The least number of shoots (1.5 ± 0.32) was obtained on 6% maltose and the least shoot length (1.2 ± 0.23) was observed on 6% glucose. Among the four types of carbon sources that were employed in the present study, fructose at 4% proved to be better choice for multiple shoot regeneration followed by sucrose, glucose and maltose from axillary bud explants of S. viarum.
Highlights
Solanum viarum (Dunal) is a much branched, prickly shrub one to two meters tall at maturity
Among the different carbohydrates used, fructose performed well followed by sucrose, glucose and maltose in terms of inducing multiple shoot number
Different types and concentration of carbon sources were used to study the effect on shoot multiplication from axillary bud explants of S. viarum
Summary
Solanum viarum (Dunal) is a much branched, prickly shrub one to two meters tall at maturity It is a member of economically important family Solanaceae. This plant grown in India as a richest source of steroids [1], which yields an economically important source of glycoalkaloide solasodine which is present in the fruit is a precursor for the steroid disogenin [2]. This is used in the synthesis of steroid hormone for treating cancer, addison’s disease, rheumatic arthritis and for providing contraceptives [3]. Solasodine though 16-Dihydropregnenolone (16-DPA) is converted to group of compounds like testosterone, methyl testosterone and corti-
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