Abstract
The energy-dependent urea permease was studied in two strains of Pseudomonas aeruginosa, measuring the uptake (transport and metabolism) of 14C-urea. In both strains urea uptake in vivo and urease activity in vitro differed significantly with respect to kinetic parameters, temperature and pH dependence and response to metabolic inhibitors. Ammonium strongly interfered both with the expression of the urea uptake system and its activity. The inhibition of the uptake activity by ammonium was partially relieved by hydraziniumsulfate, which prevented the translocation of ammonium into the cell, and in a methylammonium/ammonium transport-defective mutant of strain DSM 50071. Furthermore, methionine-sulfoximine, which prevented the intracellular glutamine formation from ammonium via inhibition of glutamine synthetase, relieved the inhibition of urea uptake by ammonium. These findings suggested that urea uptake activity in P. aeruginosa is regulated by intracellular glutamine.
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