Abstract
The function of the 5′ flanking region of the human elastin gene on transcription regulation has been investigated in chick embryo aorta cells by transient DNA transfer experiments with elastin-chloramphenicol acetyltransferase (CAT) fusions. The results have shown that the region comprised within −129 and −12 bp from the translation start site is essential for transcription and probably contains different control sequences. Expression of the reporter gene was increased 2–4-fold by addition of TGF-β to the cell cultures. Analysis of CAT expression from different deletion constructs suggests that sequences in the region −196 to −12 play a major role in TGF-β induction. The stimulating effect of the growth factor could not be observed when transfections were performed with chick embryo tendon fibroblasts. This suggests that transcriptional regulation of elastin by TGF-β is tissue specific.
Published Version
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