Abstract
Cell enrichment is currently in high demand in medical engineering. We have reported that non-blood cells can attach to a blood-compatible poly(2-methoxyethyl acrylate) (PMEA) substrate through integrin-dependent and integrin-independent mechanisms because the PMEA substrate suppresses protein adsorption. Therefore, we assumed that PMEA analogous polymers can change the contribution of integrin to cell attachment through the regulation of protein adsorption. In the present study, we investigated protein adsorption, cell attachment profiles, and attachment mechanisms on PMEA analogous polymer substrates. Additionally, we demonstrated the possibility of attachment-based cell enrichment on PMEA analogous polymer substrates. HT-1080 and MDA-MB-231 cells started to attach to poly(butyl acrylate) (PBA) and poly(tetrahydrofurfuryl acrylate) (PTHFA), on which proteins could adsorb well, within 1 h. HepG2 cells started to attach after 1 h. HT-1080, MDA-MB-231, and HepG2 cells started to attach within 30 min to PMEA, poly(2-(2-methoxyethoxy) ethyl acrylate-co-butyl acrylate) (30:70 mol%, PMe2A) and poly(2-(2-methoxyethoxy) ethoxy ethyl acrylate-co-butyl acrylate) (30:70 mol%, PMe3A), which suppress protein adsorption. Moreover, the ratio of attached cells from a cell mixture can be changed on PMEA analogous polymers. These findings suggested that PMEA analogous polymers can be used for attachment-based cell enrichment.
Highlights
Cell enrichment, a technique used to isolate or concentrate specific cells from a mixture of various types of cells, is currently in high demand because of recent developments in medicalPLOS ONE | DOI:10.1371/journal.pone.0136066 August 19, 2015Adhesion-Based Cell Enrichment on poly(2-methoxyethyl acrylate) (PMEA) Analogs engineering
The amount of protein adsorbed on the poly(tetrahydrofurfuryl acrylate) (PTHFA) substrate was approximately 20% less than that adsorbed on tissue culture polystyrene (TCPS)
We demonstrated that the number of attached HT-1080 and MDA-MB-231 cells were higher than the number of attached HepG2 cells on the poly(butyl acrylate) (PBA) and PTHFA substrates within 1 h (Fig 2A and 2B)
Summary
A technique used to isolate or concentrate specific cells from a mixture of various types of cells, is currently in high demand because of recent developments in medicalPLOS ONE | DOI:10.1371/journal.pone.0136066 August 19, 2015Adhesion-Based Cell Enrichment on PMEA Analogs engineering. Cell enrichment is expected to isolate rare cells, such as endothelial progenitor cells (EPCs) and circulating tumor cells (CTCs), from blood for tumor diagnosis and therapy of vascular failure, respectively [1, 2]. It is strongly desired for the enrichment of differentiated cells from the differentiation culture of stem cells for regenerative medical applications [3, 4]. Antibody-based enrichment requires interaction with antibodies to label the cells with high specificity. It is desirable to avoid labeling with an antibody for downstream clinical applications [7]
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