Abstract
Srpr is a gene encoding α subunit of the signal recognition particle receptor which is involved in the targeting and translocation of nascent secretory and membrane proteins to the endoplasmic reticulum. Previous studies showed aberrant expression of Srpr in several cell types with abnormal growth rate. Although Srpr is expressed in various tissues including skin, the role of Srpr in keratinocytes and regulation of its expression by miRNAs have not been studied. In this study, we investigated the role of SRPR and regulation of its expression by miRNA in skin keratinocytes. We found that SRPR was highly expressed in epidermal keratinocytes and regulated keratinocyte proliferation by affecting cell cycle progression. We also demonstrated that miR-330-5p directly inhibits Srpr expression. These data suggest that miR-330-5p-mediated regulation of the SRPR level is needed for the regulation of proliferation of epidermal keratinocytes.
Highlights
Data Availability Statement: All relevant data are within the paper and its Supporting Information files
To determine whether SRPR has a function in keratinocytes, we first examined the Srpr expression in keratinocytes and mouse dorsal skin
RT-PCR and western blot analysis revealed that both Srpr mRNA and the SRPR protein were abundantly expressed in both keratinocytes and mouse dorsal skin (Fig 1A and 1B)
Summary
Data Availability Statement: All relevant data are within the paper and its Supporting Information files. SRP binds to the signal sequence of a newly synthesized peptide and slows protein synthesis (elongation arrest) This ribosomenascent chain complex is targeted by SR to the protein-conducting channel, known as the translocon, in the endoplasmic reticulum membrane [3]. MicroRNAs (miRNAs) are non-coding RNA molecules (17–25 nucleotides) that regulate gene expression at the post-transcriptional level This regulation is mediated through the recognition and annealing of complementary sequences (referred to as ‘seed’ regions) of the miRNAs to target sites, which comprise 6–8 nucleotides in the 30 untranslated regions (UTRs) of PLOS ONE | DOI:10.1371/journal.pone.0164896. We showed that miR-330-5p directly suppresses Srpr expression by targeting its 30 UTR These data suggest that miR-3305p-mediated regulation of SRPR controls proliferation of epidermal keratinocytes
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