Regulation of regeneration and outgrowth in CNS neurons of Lymnaea is mediated by a novel EF-hand containing protein

Abstract

Event Abstract Back to Event Regulation of regeneration and outgrowth in CNS neurons of Lymnaea is mediated by a novel EF-hand containing protein Nasrin Nejatbakhsh1*, Tom Lu1, C. Guo1, R. V. Kesteren2 and Zhong-Ping Feng1 1 University of Toronto, Department of Physiology, Canada 2 Institute for Neurosciences, Department of Molecular and Cellular Neurobiology, Netherlands Neuronal injury elicits a variety of responses in the neuron. Upregulation f local protein translation in injured axons is implicated as one of the mechanisms that restores neurite integrity. The axonal or dendritic transcripts that are upregulated after injury are mostly identified as structural and/or structural related proteins in both vertebrates and invertebrates. We recently identified the transcripts of a novel calcium binding protein, LCaBP, in the neurites and growth cones of Lymnaea central neurons. LCaBP is encoded with putative EF-hands motifs. In this study we investigated the role of local LCaBP synthesis in neurite regeneration in cultured PeA cells. Using in situ hybridization, we demonstrated that LCaBP transcripts are distributed in both the soma and growth cone regions of cultured PeA cells. We also demonstrated that LCaBP protein is ubiquitously expressed in the soma, neurites and growth cones of cells using the specific LCaBP antibody that we recently constructed. Using gene silencing approach, we showed that reduction of LCaBP protein by LCaBP siRNA, not the control sRNA, resulted in a decrease in neurite outgrowth in both intact neurites and neurites which had undergone axotomy. In addition, we observed increases in neurite elongation in the control neurons 3 hours after axotomy. However, all neurites in cells treated with LCaBP siRNA exhibited retraction following axotomy during the same time course. These findings indicate that LCaBP plays a critical role in the elongation of intact neurites and the regeneration and continued elongation of injured neurites. Conference: B.R.A.I.N. platform in Physiology poster day 2009, Toronto, ON, Canada, 16 Dec - 16 Dec, 2009. Presentation Type: Poster Presentation Topic: Poster presentations Citation: Nejatbakhsh N, Lu T, Guo C, Kesteren RV and Feng Z (2009). Regulation of regeneration and outgrowth in CNS neurons of Lymnaea is mediated by a novel EF-hand containing protein. Front. Neurosci. Conference Abstract: B.R.A.I.N. platform in Physiology poster day 2009. doi: 10.3389/conf.neuro.03.2009.17.039 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Dec 2009; Published Online: 18 Dec 2009. * Correspondence: Nasrin Nejatbakhsh, University of Toronto, Department of Physiology, Toronto, Canada, nasrin14@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nasrin Nejatbakhsh Tom Lu C. Guo R. V Kesteren Zhong-Ping Feng Google Nasrin Nejatbakhsh Tom Lu C. Guo R. V Kesteren Zhong-Ping Feng Google Scholar Nasrin Nejatbakhsh Tom Lu C. Guo R. V Kesteren Zhong-Ping Feng PubMed Nasrin Nejatbakhsh Tom Lu C. Guo R. V Kesteren Zhong-Ping Feng Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.