Abstract
Summary Translational fusions of nitrogen fixation ( nif ) genes to the β-galactosidase ( lacZ ) gene are used to investigate nif gene regulation, in response to different nitrogen sources and oxygen concentrations, to free living nitrogen fixing cultures of Azorhizobium caulinodans ORS571. Derepression of nif gene promoters is also examined in nitrogen fixing stem- and root nodules induced on Sesbania rostrata . The effect of increasing nitrate concentrations on nodulation and nitrogen fixation is determined. These results are reviewed in the light of recent progress in understanding the structure, function and regulation of nitrogen fixation and assimilation genes of A. caulinodans ORS571.
Published Version
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