Abstract
Human mature eosinophils have been cultured for up to 2 weeks by coculture with endothelial cells, or by culture in enriched medium supplemented with interleukin 3, interleukin 5, granulocyte/macrophage colony-stimulating factor, or the serum of a patient with the idiopathic hypereosinophilic syndrome. Compared to the freshly isolated eosinophils, the cultured eosinophils underwent phenotypic changes characterized by enhanced calcium ionophore-induced leukotriene C4 generation, enhanced cytotoxicity for antibody-coated larvae of Schistosoma mansoni and hypodensity as defined by sedimentation analysis. These findings and an integrated view of the regulation of eosinophil post-mitotic phenotype are presented.
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