Abstract
The liver maintains metabolic homeostasis by integrating the regulation of nutrient status with both hormonal and neural signals. Many studies on hepatic signaling in response to nutrients have been conducted in mice. However, no in-depth study is currently available that has investigated genome-wide changes in gene expression during the normal physiological fasting-feeding cycle in nutrient-sensitive and -insensitive mice. Using two strains of mice, C57BL/6J and BALB/cJ, and deploying deep RNA-Seq complemented with quantitative RT-PCR, we found that feeding causes substantial and transient changes in gene expression in the livers of both mouse strains. The majority of significantly changed transcripts fell within the areas of biological regulation and cellular and metabolic processes. Among the metabolisms of three major types of macronutrients (i.e. carbohydrates, proteins, and lipids), feeding affected lipid metabolism the most. We also noted that the C57BL/6J and BALB/cJ mice significantly differed in gene expression and in changes in gene expression in response to feeding. In both fasted and fed states, both mouse strains shared common expression patterns for about 10,200 genes, and an additional 400-600 genes were differentially regulated in one strain but not the other. Among the shared genes, more lipogenic genes were induced upon feeding in BABL/cJ than in C57BL/6J mice. In contrast, in the population of differentially enriched genes, C57BL/6J mice expressed more genes involved in lipid metabolism than BALB/cJ mice. In summary, these results reveal that the two mouse strains used here exhibit several differences in feeding-induced hepatic responses in gene expression, especially in lipogenic genes.
Highlights
The liver maintains metabolic homeostasis by integrating the regulation of nutrient status with both hormonal and neural signals
Most transcriptomic studies in the liver have used the C57BL/6J mice that are relatively sensitive to dietinduced obesity and compared steady-state differences between mice maintained on a low-fat or high-fat diet [5,6,7,8,9]
A few studies have examined other strains of mice that are relatively resistant to diet-induced obesity (e.g. BALB/cJ mice) on low-fat versus high-fat diets [6, 10, 11], there have not been any direct comparisons between strains or systematic analyses of the temporal changes in gene expression during the normal physiologic fasting-feeding cycle
Summary
In this study, there were eight groups with five mouse replicates for each group, resulting in a total of 40 liver samples from 40 individual mice. The RNA-Seq data for all samples have been deposited in the GEO Database with accession number GSE 137385. The quality of mRNA-Seq data from the 40 samples is summarized, with error rates for all samples Ͻ0.03% and Ͼ90% of reads with Q30. The reproducibility and reliability of these data sets are demonstrated, showing that gene expression reads as fragments per.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
