Regulation of enzymatic and non-enzymatic antioxidants in rice seedlings against chromium stress through sodium hydrosulfide and sodium nitroprusside.

Abstract

Hydrogen sulfide (H2S) and nitric oxide (NO) play a well-organized protective mechanism in coping with oxidative stress induced by toxic metals. However, the comparative effects of H2S and NO on enzymatic and non-enzymatic antioxidants in plants under Cr(III) stress have not been defined. In this study, we mathematically evaluate the importance of sodium hydrosulfide (NaHS) and sodium nitroprusside (SNP) on these two antioxidant systems in rice seedlings under Cr(III) stress. The results displayed that the optimal dose of NaHS and SNP was 100μM and 75μM, respectively, in rice tissues under Cr(III) stress, judging by the reactive oxygen species (ROS) levels in rice tissues. When rice seedlings wereexposed to Cr(III) atconcentrations of3.57, 7.24, 26.52mg Cr/l, Cr-inducedROS accumulationhad a significant (p < 0.05)effecton the lipid peroxidation of cell membrane in rice tissues, and decreasedthe response of SOD, CAT, Pro, GSH, and AsA in rice tissues. Application of exogenous NaHS and SNP effectively (p < 0.05) alleviated the toxic effects of Cr(III) in rice seedlings by activating different antioxidants. A novel physiological response model suggested that the regulatory effect of NaHS and SNP on non-enzymatic antioxidants was stronger than that of enzymatic antioxidants. Moreover, NaHS-mediated response intensity of both enzymatic and non-enzymatic antioxidants was greater than that of SNP, suggesting the importance of utilizing NaHS over SNP as antioxidant regulators during detoxification of Cr(III) in rice seedlings.