Abstract
Background In order to develop treatments or preventive measures for auditory hair cell loss, an understanding of both the process of auditory hair cell regeneration and factors that influence this process, is needed. Our previous microarray analysis showed that growth hormone (GH) was significantly upregulated during zebrafish auditory hair cell regeneration, coupled with cell proliferation [1,2]. We further tested the effects of GH on zebrafish auditory hair cell regeneration by injecting GH after sound exposure and found that GH can efficiently promote post-trauma auditory hair cell regeneration, which may be achieved through stimulating proliferation and suppressing apoptosis [3]. In the current study, we used Next Generation Sequencing (NGS) to examine the possible GH pathways involved in zebrafish auditory hair cell regeneration.
Highlights
In order to develop treatments or preventive measures for auditory hair cell loss, an understanding of both the process of auditory hair cell regeneration and factors that influence this process, is needed
Genes associated with cellular growth and proliferation, cell cycle, cell death, cancer, tissue development, and neurological disease were highly regulated in our data sets comparing baseline to buffer-injected fish, bufferinjected to antagonist-injected fish, and buffer-injected to growth hormone (GH)-injected fish
We further examined the effect of GH by analyzing the GH receptor pathway
Summary
In order to develop treatments or preventive measures for auditory hair cell loss, an understanding of both the process of auditory hair cell regeneration and factors that influence this process, is needed. Materials and methods Groups of 20 zebrafish were exposed to a 150 Hz tone at 179 dB re 1 μPa RMS for 40 h. The fish were injected with either phosphate buffer, GH, or a GH antagonist. One baseline group received no acoustic stimulus or injection.
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