Abstract
Background While acoustic trauma results in a loss of hair cells in the ears of fishes, they have the ability to recover their hair cells and hearing sensitivity within a few weeks [1]. Our previous microarray and RT-PCR analysis of sound-exposed zebrafish (Danio rerio) ears, showed that growth hormone (GH) was significantly upregulated during zebrafish auditory tissue cell proliferation and hair cell regeneration [2]. This upregulation was greatest two days following acoustic trauma, coincident with an increase in cell proliferation [3]. In order to better understand the role of GH in the regenerative abilities of the zebrafish ear, we performed two GH-injection experiments.
Highlights
While acoustic trauma results in a loss of hair cells in the ears of fishes, they have the ability to recover their hair cells and hearing sensitivity within a few weeks [1]
growth hormone (GH) injection resulted in increased cell proliferation in the zebrafish ear, in the utricle (Fig. 1A)
48 h post-sound exposure, the saccules, lagenae, and utricles of GH-injected fish had significantly reduced BrdU-labeled cells compared to controls (Fig. 1B)
Summary
While acoustic trauma results in a loss of hair cells in the ears of fishes, they have the ability to recover their hair cells and hearing sensitivity within a few weeks [1]. Materials and methods In Experiment 1, treatment fish were injected intraperitoneally with salmon GH at 10 ug/1g body weight while controls were injected with buffer solution. Both groups were allowed to recover for 24 h at 25 oC before
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