Regulation of Autophagy and Inflammation Improves the Corneal Injury in the Model of Rats with Xerophthalmia

Abstract

This study assessed the mechanism of regulation of autophagy and inflammation on corneal injury in the model of rats with xerophthalmia. The level of inducer and inhibitor of autophagy in the model of rats with xerophthalmia was detected and cell proliferation was evaluated by MTT assay together with analysis of colony formation, cell apoptosis and cycle by FCM. The effect of inducer on the corneal injury and inflammation was assessed. The level of autophagy marker LC3 was elevated significantly after treatment with autophagy inducer along with increased cell proliferation and migration and strengthened sensibility of corneal epithelial cells on corneal injury and inflammation and autophagy rate. In addition, cells in the established model was blocked at G2/M phase. Moreover, autophagy inducer significantly upregulated MMP-10 expression. Furthermore, there was a target relationship between LC3 and P62. In conclusion, the cell migration, growth and autophagy is induced with autophagy inducer in the model of rats with xerophthalmia, indicating that autophagy inducer might be a brand-new therapeutic target spot for the treatment of xerophthalmia.