Abstract
Hepatic albumin synthesis is down-regulated after both inflammation and hepatectomy. The transcriptional control of albumin synthesis was investigated in models of both conditions to differentiate the underlying mechanisms. Male Donryu rats underwent 70% hepatectomy or turpentine injection. Serum albumin and mRNA levels of albumin and promoter binding proteins (D site binding protein [DBP] CCAAT/enhancer binding protein-α[ C EBP -α], -β , and hepatocyte nuclear factor-1 [HNF-1]) in the liver were measured from 0 to 96 hr. After hepatectomy, the albumin mRNA level decreased to 0.6 at 36 hr and then recovered. After turpentine injection, it decreased to 0.4 at 36 hr and then recovered. The serum level of albumin decreased in a time-dependent manner in both models. The C EBP -α mRNA level decreased to 0.5 at 6 and 12 hr after hepatectomy and to 0.6 at 24 hr after turpentine injection. The DBP mRNA level decreased to 0.3 at 6 hr, to 0.2 at 24 hr after hepatectomy, and to 0.3 at 30 hr after turpentine injection. The C EBP -β mRNA level increased to 1.7 at 3 hr after hepatectomy and to 1.5 at 12 hr after turpentine injection. On the other hand, HNF-1 mRNA levels showed no consistent change in either model. The change in mRNA of the nuclear factors ( C EBP -α, C EBP -β , and DBP) thus precedes that of albumin. In conclusion, transcriptional regulation of albumin synthesis in the regenerating and the acute inflammation phase of the liver can be assessed by monitoring the mRNA levels of nuclear factors. The mechanisms for down-regulation of albumin in both conditions share substantial similarities.
Published Version
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