Abstract
The regulation by phosphate of abscisic acid (ABA) production by Mycosphaerella (Cercospora) rosicola was examined in defined media, submerged shake cultures. Phosphate concentrations below 0.5 mM were suboptimal for growth and caused ABA synthesis to begin earlier than at 0.5 mM, the optimal concentration for maximal yield of ABA. Higher concentrations of phosphate gave progressively greater suppression of ABA formation while dry weight yield was essentially constant from 0.5 mM to 10 mM. Addition of 5 mM phosphate to phosphate-limited cultures at various times showed that there was a critical period during the first 3–5 da of incubation during logarithmic growth and before ABA synthesis had begun when the fungus was sensitive to phosphate suppression.
Published Version
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