REGULARITIES OF PROTEOLYSIS OF PACKED LIVESTOCK RED BLOOD CELLS

Abstract

Actual methods for anti-anemic preparation producing are characterized by a number of weak points: significant hemoglobin losses during hemolysis; difficulties in achieving a high hemoglobin purity level; expensive and complex instrumentation etc. A promising direction of this research is a study of a possibility to get iron-containing mass from meat-producing animals’ blood through hydrolysis with acetic and citric acids. The author studies a correlation dependence of acid concentration on amine nitrogen mass fraction, heme iron, hydrolysis level. For packed pork blood cells, the highest amine nitrogen values are typical under the use of acetic and citric acids with mass fraction of 10%, which corresponds to 357 mg/100 cm3 under the duration of 10 hours for citric acid and 305 mg/100 cm3 under the duration of 8 hours for acetic acid. Under packed cattle blood cells hydrolysis, the highest amine nitrogen values are typical under the use of 10% citric acid solution and 10% acetic acid solution, which corresponds to 268 mg/100 cm3 i 229 mg/100 cm3 respectively. Iron yield has positive increasing dynamics. 11-12 hour hydrolysis is relatively stable, the changes in iron content are insignificant, which means that hydrolysis time increase makes no sense. Favourable conditions for hydrolysis are: mass fraction of citric acid - 10%, mass fraction of citric acid - 5%, mass fraction of acetic acid - 10%, temperature - 50 ± 1°C, process time - 12 hours.