Abstract
CCAAT displacement protein (CDP) is a putative repressor of tissue-specific gene expression, implicated in regulation of expression of the human myeloid-specific gene, gp91-phox. The cDNA sequence of the human gene predicts a polypeptide of 1506 amino acids, with a homeodomain and three repeated motifs closely homologous to cut, a protein known to specify the cell fate of peripheral neurons and other tissues during embryonic development of Drosophila. Therefore, the human gene locus for CDP has been named CUTL1, for cut-like 1. Preliminary data revealed that CUTL1 mapped to human chromosome 7 (E.J.N., P.M.-J.L., and S.H.O., unpublished results). Since diseases with abnormalities of myeloid differentitation often have aberrations of chromosome 7, especially in patients previously exposed to mutagenic agents or radiation therapy, the cell-type specificity of CDP makes it an attractive candidate for involvement in these conditions. As a first step in addressing this question, we have examined the regional localization of CUTL1 using a panel of rodent-human somatic cell hybrids containing various portions of chromosome 7. The results of our hybridization analysis are summarized. The regional localization of CUTL1 to 7q22 was based primarily on the negative hybridization signal with the human/hamster hybrid 1EF2/3/K017 (7cen-q21) and the positive result withmore » 1CF2/5/K016 (7cen-q22). Since no hybridization signal was detected in GM1059Rag5 (a human-mouse hybrid carrying a single human chromosome 7 with an interstitial deletion 7pter-q22:q32-qter) and 2068Rag22-2 (7qter-q22), the regional assignment of CUTL1 could be narrowed to the distal boundary of 7q22.« less
Published Version
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