Regional assignment of human alcohol dehydrogenase (ADH) gene to 4pter----4q21.

Abstract

The hybrid clone FD1 constructed by fusion of Chinese hamster cell line Wg3-h with human lymphocyte was irradiated with X-ray. Fourteen survival clones were isolated and 3 of them, F5B, F52B, F61A were analyzed in detail by cytogenetic and biochemical methods. The results of chromosome G-banding followed by Giemsa-11 differential staining show that there exists a deleted human chromosome 4 in all of the three hybrids. This deletion of human chromosome 4 in F61A is 4pter----4q21. The results of isozyme analysis of phosphoglucomutase-2 (PGM2) which is located on 4p14----4q21 confirm our cytogenetic conclusion. We used polyacrylamide gel electrophoresis to study the alcohol dehydrogenase (ADH) in human lymphocyte, Wg3-h and hybrid clones. Their electrophoretic pattern showed that human ADH isozyme did express, in the peripheral blood lymphocyte, hybrids F5B, F52B, F61A and FD1. According to these results, we suggest that one of the Class I ADH structural genes is located on the human chromosome 4pter----4q21. Recently, McKusick reported that Class I ADH gene cluster had been assigned to chromosome 4q21----4qter. Considering this fact, we suggest that Class I ADH gene might be assigned to chromosome 4q21 or on its vicinity.