Molecular genetic studies on alcohol and aldehyde dehydrogenase: individual variation, gene mapping and analysis of regulation.

Moyra Smith

doi:10.1042/bst0160227

Abstract

Molecular genetic studies on alcohol and aldehyde dehydrogenase: individual variation, gene mapping and analysis of regulation.

Highlights

Title Molecular genetic studies on alcohol and aldehyde dehydrogenase: individual variation, gene mapping and analysis of regulation
Refinement of purification procedures led to isolation of enzyme polypeptides suitable for amino-acid sequence analysis (Impraim el a/., 1982; Hempel et a/., 1984a,h,c; Buhler et ul., 1984).Availability of amino-sequence data led to the development of oligonucleotide probes to screen liver complementary (c)DNA libraries to isolate cDNA clones for alcohol and aldehyde dehydrogenases (Duester et a/., 1984; Smith, 1986; von Bahr-Lindstrom et a/., 1986)
Analysis of RNA species in different tissues and detailed analysis of cDNA clones has led to insights into some of the factors which may be involved in the regulation of expression of class 1 alcohol dehydrogenases and mitochondrial aldehyde dehydrogenases (ALDH)

Summary

625th MEETING, LONDON von Bahr-Lindstrom, H., Jeck, R., Woenckhaus, C., Sohn, S., Hempel. Molecular genetic studies on alcohol and aldehyde dehydrogenase: individual variation, gene mapping and analysis of regulation. Refinement of purification procedures led to isolation of enzyme polypeptides suitable for amino-acid sequence analysis (Impraim el a/., 1982; Hempel et a/., 1984a,h,c; Buhler et ul., 1984).Availability of amino-sequence data led to the development of oligonucleotide probes to screen liver complementary (c)DNA libraries to isolate cDNA clones for alcohol and aldehyde dehydrogenases (Duester et a/., 1984; Smith, 1986; von Bahr-Lindstrom et a/., 1986). Analysis of DNA from hybrids containing fragments of human chromosome 4, provided evidence that the class 1 ADH genes were located in the region 4q21-4q24 (Smith et a/., 1985).Confirmation of this physical assignment has been obtained in studies on the linkage relationships of ADH and other genes assigned to human chromosome 4 (see below)

Derivation of A D H genomic clones

No of chromosomes