Abstract
Cotyledonary, leaf, hypocotyl, and shoot-tip explants of mulberry (Morus alba L.), derived from embryos cultured with benzyladenine (BA), were induced to form adventitious shoots when cultured in media containing high BA with or without a low concentration of naphthaleneacetic acid (NAA). The adventitious shoots formed at the basal cut ends of the cotyledons and leaves. Histological analysis of the explants showed a wound cambium at the base of the bud primordia. In addition to adventitious buds, shoot-tip explants also produced axillary buds. Higher levels of NAA in the medium completely nullified the effectiveness of BA. Elongation of the buds was achieved by gradual reduction of BA in the medium. Plantlets were regenerated by inducing root formation on the shoots with indole-3-butyric acid.
Published Version
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