Regeneration of Indica Rice ( Oryza sativa L.) from Primary Callus Derived from Immature Embryos

Abstract

Summary To identify important parameters controlling the regeneration of indica rice (Oryza sativa L.) callus, immature embryos of IR54 were plated onto callus induction medium consisting of either MS or N6 basal salts. Various medium supplements were subsequently compared for ability to induce callus growth and plant regeneration. It was found that callus growth and plant regeneration were influenced by organic supplements to the basal media, medium solidifying agents, the number of embryo-derived calli per plate during plant regeneration, and phytohormone concentration during callus induction. However, these factors often influenced regeneration in contrasting ways depending on the basal medium used. For optimum plant regeneration N6 callus induction medium was supplemented with 1 g 1 -1 casein hydrolysate, 0.2 % (w/v) Gelrite, and 0.5 mg -1 -1 2,4-D. Regeneration was accomplished by subculturing one callus per plate to identical medium lacking 2,4-D.