Abstract
In an earlier paper, we described a procedure for the isolation of glutamine synthetase and the protein product of the groE gene (p groE) by polyethyleneimine precipitation and affinity chromatography on a Blue Dextran column ( Z. F. Burton and D. Eisenberg, 1980, Arch. Biochem. Biophys. 205, 478–488 ). Subsequently we found that several of the steps can be omitted when isolating glutamine synthetase. Two procedures are described which are very rapid and quantitative for the recovery of glutamine synthetase activity and which are useful for handling quantities of cells at least up to 500 g.
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