Abstract
Information on glutamine synthetase (GS) activity of roots and leaves in response to nitrogen (N) application at different growth stages is limited for field‐grown rice. Root and leaf GS activity was measured on field‐grown rice plants to compare the effects of fertilizer‐N application at midtillering, panicle initiation, and flowering. Leaf GS activity was greater than root GS activity, regardless of N application. Root and leaf GS activity generally declined as plant aged, and the decline was greater in roots than leaves. Leaf and root GS activity were significantly increased by urea‐N applied at different growth stages. Root GS activity was maximum three days after N application, while leaf GS activity peaked one day after N application. Root GS activity showed greater response to N application at midtillering and panicle initiation than leaf GS activity, but the opposite was found at flowering. The stimulation of GS activity by N was greatest at midtillering and reduced with plant age.
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