Reduction of Oxidative Stress and Testicular Tissue Damage in Wistar Rat Treated with Diazinon by Quercetin

Abstract

Background: Diazinon is an organophosphorus compound widely used as a pesticide in agriculture. It causes oxidative stress and has toxic effects on the reproductive system. Quercetin is a carotenoid with antioxidant activity. Objectives: This research aimed to evaluate quercetin's protective effects on oxidative stress and reduce testicular tissue damage in rats treated with diazinon. Methods: In the present study, 40 adult Wistar rats were divided into five groups. Each group had eight rat, including control-sham (0.2 mg/kg/B.W of corn oil), diazinon (300 mg/kg/B.W), experimental 1; diazinon (300 mg/kg/B.W) and quercetin (25 mg/kgB.W), experimental 2; diazinon (300 mg/kg/B.W) and quercetin (50 mg/kgB.W), experimental 3; diazinon (300 mg/kg/B.W) and quercetin (100 mg/kgB.W). The rats were given orally daily for two months. Results: According to the results, diazinon decreased repopulation index (RI), FRAP testosterone levels, and carbohydrate reserves and increased malondialdehyde (MDA), sperm index (SI), negative tubule differentiation index (TDI), and lipid reserves in the testicular tissue of rats compared to the control sham. All the above parameters in the diazinon group with quercetin were returned to near normal. Conclusions: Based on the results, diazinon negatively affected the histochemical and histology of the testis in the Wistar rat. The rat's reproductive system is protected against diazinon if combined with quercetin.