Abstract
Background: To investigate disturbances in fibrinolytic components in diabetic rats with middle cerebral artery occlusion. Methods: Middle cerebral artery occlusion in diabetic (induced by streptozotocin ) and control rats was performed using intraluminal procedure. Cerebral infarction volume was detected with TTC staining. The mRNA of tissue plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA), plasminogen activator-1 (PAI-1), neuroserpin (NSP) was detected with RT-PCR. PA and PAI activity was determined with spectrophotometric methods. Results: Comparison of cerebral injury at 23 hours after reperfusion indicated that infarction volumes were increased in diabetic rats as compared to normal rats. Cerebral ischemia/reperfusion in normal and diabetic rats was accompanied by increased expression of t-PA, u-PA, PAI-1 and NSP mRNA at 1, 2, 5, 11, and 23 hours after reperfusion. Expression of NSP mRNA, but not t-PA, u-PA and PAI-1 mRNA, reduced to undetectable levels at 11 and 23 hours after reperfusion in diabetic rats, compared to normal rats. In ischemic brains of diabetic rats and normal rats, activity of PA and ratio of PA/PAI was significantly reduced at 5 hours after reperfusion, and that of diabetic rats reduced more. Conclusion: The exacerbation of ischemic injury in diabetic rats may related to reduction of fibrinolytic components and neuroprotective role of NSP.
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