Abstract
Superfusion of guinea pig liver slices with a solution containing dehydro-epiandrosterone sulfate resulted in the formation of 5-androstene-3β,17β-diol sulfate. When ethanol, 2.2–43.5 mM, was added to the superfusion fluid, there was a consistent increase (between 1.3 and 4.5 times) in androstenediol sulfate production. The same magnitude of increase occurred during superfusion with 1,1-dideuteroethanol. In addition, there was a prompt incorporation of deuterium into the androstenediol sulfate to a maximum of a 42 atom percent excess. In the presence of 4-bromopyrazole, an inhibitor of alcohol dehydrogenase (alcohol:NAD oxidoreductase, EC 1.1.1.1), dideuteroethanol did not bring about an increase in deuterated or nondeuterated androstenediol sulfate production. It is suggested that the increased concentration of NADH during ethanol metabolism caused an increased reduction of the 17-ketosteroid to the 17β-hydroxysteroid. The results offer an explanation for the increase of the plasma concentration of 17β-hydroxysteroid sulfates observed after administration of alcohol to humans.
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