Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation

Abstract

BackgroundAn increased prevalence of allergic disorders in developed countries has been associated with decreased exposure to environmental micro-organisms and an alteration of microbiota colonization. An appropriate model is needed to investigate the mechanisms by which hygiene environment-driven changes in microbiota could regulate allergic disorders. ObjectiveTo discover the correlation between the higher incidence and severity of allergies with the relative hygiene environment in a developed country. MethodsAllergic respiratory inflammation was induced in specific pathogen-free and control rats by sensitization and challenge with ovalbumin. The diversity of lower airway bacteria community was analyzed by polymerase chain reaction denaturing gradient gel electrophoresis and sequencing before ovalbumin sensitization. Allergic respiratory inflammation resulting in cellular infiltrate was measured after the last challenge. ResultsThe diversity of microbiota in the airway of specific pathogen-free rats decreased compared with the control rats; the more frequent microbiota in the control rats were Proteobacteria and Bacteroidetes. In addition, increased nasal rubbing and sneezing combined with exaggerated IgE production and leukocyte number was observed in ovalbumin-treated specific pathogen-free rats. ConclusionThese data indicate that the excessive “hygienic” environment resulted in a decreased bacterial diversity in the airway during infancy, leading to an increased susceptibility to allergic disease.