Abstract
The lambda Red recombination system works poorly among unreplicated gam+ lambda chromosomes in recA- cells compared to recA+ cells. Recombination is not enhanced in recA- recB-cells. Thus, the inability of Red to promote recombination in recA- replication-blocked cross is not due to the hypothetical destruction of recombination intermediates by the recB nuclease. This conclusion strengthens previous proposals that the products of the red genes can operate upon recombinational intermediates which require recA activity for their formation.
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