Recurrent somatic embryogenesis and plant regeneration from seedlings of Hepatica nobilis Schreb.

Bożena Szewczyk-Taranek,Bożena Pawłowska

doi:10.1007/s11240-014-0661-7

Bożena Szewczyk-Taranek, Bożena Pawłowska

Open Access

https://doi.org/10.1007/s11240-014-0661-7

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Abstract

A method for secondary somatic embryogenesis was developed on embryos derived from embryogenic callus formed on Hepatica nobilis seedlings. Somatic embryogenesis (SE) was induced on seedlings (on the hypocotyl and epicotyl parts) grown on the Murashige and Skoog (1962) medium (MS) supplemented with 1 µM naphthaleneacetic acid (NAA), and/or 0.1 µM 6-benzyladenine (BA) and on medium without plant growth regulators (PGR). The best response of embryogenic callus formation was observed on the medium containing 1 µM NAA alone or with 0.1 µM BA. Individual somatic embryos, formed on embryogenic callus on the medium without PGR (MS0), at heart, torpedo and cotyledonary stage, were transferred to the media where secondary somatic embryo formation and development into plantlets occurred. Although the most efficient repetitive cycles of secondary SE were recorded for all stages of somatic embryos (heart, torpedo, cotyledonary) on the MS0 medium (77.8–87.4 %), secondary somatic embryos were also obtained on all media supplemented with cytokinins. The best rate of somatic embryos germination was achieved on MS media with 0.2 µM NAA and 2 µM BA, and 0.1 µM NAA and 1 µM BA (48.8–52.0 %) when more mature embryos (cotyledonary stage) were used. Plantlets grown from somatic embryos were successfully acclimatized to greenhouse conditions.

Highlights

The genus Hepatica Mill belongs to the family Ranunculaceae and includes ten species of evergreen hemicryptophytes found in deciduous forests of the Northern Hemisphere
A method for secondary somatic embryogenesis was developed on embryos derived from embryogenic callus formed on Hepatica nobilis seedlings
The most efficient repetitive cycles of secondary Somatic embryogenesis (SE) were recorded for all stages of somatic embryos on the MS0 medium (77.8–87.4 %), secondary somatic embryos were obtained on all media supplemented with cytokinins

Summary

Introduction

The genus Hepatica Mill belongs to the family Ranunculaceae and includes ten species of evergreen hemicryptophytes found in deciduous forests of the Northern Hemisphere. Japonica Nakai) with various flower forms, petal colours and foliage types, are the effect of the Japanese breeding programs and are among the most expensive perennials commercially available (Hinkley 2009). It is a very valuable perennial for horticulture and landscape architecture due to the shade tolerance, early spring blooming and decorative evergreen leaves. Leaves from in vitro plants of H. nobilis were used to stimulate adventitious bud formation (Szewczyk-Taranek and Pindel 2009).

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