Rectal Firmicutes, Actinobacteria and Gammaproteobacteria phyla fitness belong to post mortem intervals in the terrestrial environment death victims evaluation in Malang, East Java, Indonesia

Tengku Putri Sholihah,Husnul Khotimah,Noorhamdani Noorhamdani,Eriko Prawestiningtyas

doi:10.30574/gscbps.2019.6.3.0001

Tengku Putri Sholihah, Husnul Khotimah + Show 2 more

Open Access

https://doi.org/10.30574/gscbps.2019.6.3.0001

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Abstract

The increasing of urbanization will lead the increasing violence-related death, like traffic accident, murdered and suicide which contribute to 20 leading causes of death. Post mortem interval (PMI) estimation is important information for suspicious death. Microbes have important role for decomposition of bodies. By evaluate the certain bacteria will know the post mortem interval because the community of bacteria have certain condition regarding to the composition. The aim of this study is to figure out the community of Firmicutes, Actinobacteria and Gammaproteobacteria from rectal swab post mortem interval of the terrestrial environment in Malang, East Java, Indonesia. We used Wistar carcass as a model for human decomposition. Identification of bacterial phyla using Real Time PCR (qPCR) method after cultured in nutrient broth solution. The results showed that Firmicutes has dominant composition in fresh stage (0-24 hours) and in advance decomposition at 120 hours after death. Actinobacteria was dominant in the early decomposition, observed from the 48 to 72 hours after death. While Gammaproteobacteria was dominant in the end of fresh stage and early decomposition stage when bloating time occur.

Highlights

The trend of urbanization, especially in Asia and Africa, has led to the violence development problems that are concentrated in the urban centers of developing countries [1]
The increasing of urbanization will lead the increasing violence-related death, like traffic accident, murdered and suicide which contribute to 20 leading causes of death
The aim of this study is to figure out the community of Firmicutes, Actinobacteria and Gammaproteobacteria from rectal swab post mortem interval of the terrestrial environment in Malang, East Java, Indonesia

Summary

Introduction

The trend of urbanization, especially in Asia and Africa, has led to the violence development problems that are concentrated in the urban centers of developing countries [1]. Cases of violence-related deaths, like traffic accidents, murder and suicide, are expected can contribute to increased compared to other causes of death and are among the 20 leading causes of death in the world by 2030 [2]. PMI estimation depend on physical changes that occur after death, such as algor mortis, livor mortis, rigor mortis and supravital activity [4, 5]. This method cannot be used to the advanced post-mortem period, because it only provides a general estimate of PMI. Additional techniques have been developed for estimating PMI such as thanatochemistry [6], DNA / RNA degradation [7, 8] and forensic entomology [9, 10]

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