Abstract
Nisin is a class I bacteriocin, which is produced by lactic acid bacteria (LAB) belonging to Lactococcus and Streptococcus genera. Nisin is approved by the FDA (Food and Drug Administration) as a natural biopreservative agent under reference E234. Besides food application, nisin has also a potential for therapeutic application to treat a range of infectious diseases. Nisin, which is commercially available, is mainly produced by liquid fermentation. The production of commercial high-quality nisin is hampered by the high cost of downstream processing, which often involves subsequent steps of salt precipitation, centrifugation, and chromatography. Membrane processes offer a set of advantages. Indeed, these processes are simple and effective technologies for protein concentration and separation; One of the biggest limitations is the decrease in permeate flux. In this study, the performance of three UF membranes (10, 50, and 100 kDa) for the separation and concentration of nisin obtained from Lactococcus lactis (L. lactis) culture supernatants was evaluated. Permeate flux decline, fouling resistances, fouling index, nisin recovery in the permeate and retentate streams were evaluated. The 10 kDa membrane showed the best nisin recovery properties with the highest recovery yield and purification factor, achieving 100% and 4 in the retentate stream, respectively.
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