Abstract
The endo-glucanase (E.C. 3.2.1.4) was produced by Aspergillus terreus adopting solid state fermentation (SSF) using agro residues as main substrate. To recover the enzyme from the fermented mass, different extraction liquids were tried and 10% aqueous solution of glycerol was found to be superior. When the selected extractant was applied at different ratio to the fermented solid mass, maximum enzyme was recovered at 1:5 (w/v) ratio. The other process parameters (time, temperature and mixing speed) effects on the enzyme recovery were subsequently studied by response surface methodology (RSM). Box-Bhenken Design of experiment (BBDOE) was exploited for the analysis of interactive effects of the independent variables. The optimization was done following the numerical approach focusing reduction in utility cost without compromising the endo-glucanse activity. Based on the predicted solution the validation experiments were carried out and finally 32 IU/g of endo-glucanase was recovered at room temperature, at a mixing speed of 100 rpm in 2.65 h which was very close to the predicted response. The optimization evidenced more than two times betterment in enzyme recovery than the un-optimized state. The model developed was found to be robust for process analysis. Repetitive extraction had revealed that maximum endo-glucanase recovery was required of two cycles of extraction at optimized conditions.
Highlights
Cellulose, the most abundant natural renewable biopolymer in the earth, is commonly degraded in to smaller fragments and glucose by the hydrolytic action of cellulase
solid state fermentation (SSF) is a process wherein fermentation has been done in the absence of free liquid and first stage of product recovery from the fermented mass is mainly done by applying suitable extraction liquid to leach out the protein in the liquid phase for further processing
For further refinement different concentrations of glycerol were employed but marginal improvement was obtained for the rest of the study 10% aqueous soln. of glycerol had been used as extraction liquid
Summary
The most abundant natural renewable biopolymer in the earth, is commonly degraded in to smaller fragments and glucose by the hydrolytic action of cellulase. Cellulases are industrially produced by submerged process but solid state fermentation (SSF) exploited and reported based on its economics and other advantages over submerged fermentation (SmF) described in different reports (Acuña-argüelles et al, 1995; Medina-Morales et al, 2011; Trulea et al, 2013; Sarao et al, 2013; Toor et al, 2013; Saida et al, 2013; Sharma et al, 2013). There are several reports on the optimization of recovery of different enzymes produced by SSF root (Rodríguez et al, 2012, Aikat et al, 2000, Ahmed et al 2013, Palit et al, 2001, Shata et al, 2012) but on the endo-glucanase very few systematic studies have been found (Chandra et al, 2010). The present investigation was designed to study the interactive effects of environmental conditions on the recovery of endo-glucanase from the fermented mass using RSM and its statistical optimization
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of microbiology, biotechnology and food sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
