Abstract
White-rot basidiomycetes have evolved as efficient degraders of wood. These fungi produce the range of degradative enzymes neccessary to depolymerize its complicated structure. Although they have potential use in a variety of applications, most of the enzymes from these fungi are not commercially available. Those available are typically impure, or being only minor activities of non-specific enzymes and are frequently misidentified. Due to the characteristics of solid substrate fermentations, the concentration of enzymes produced by fungi are often higher than those obtained in liquid fermentations. Spent fungal cultures available from commercial mushroom farms provides a potentially abundant source of low-cost material for extraction. Recovery of enzymes from such cultures can provide a synergistic set of degradative enzymes in quantities sufficient to dvelopment of new industrial applications. Summarized are our initial studies concerning the recovery of enzymes from commercial shiitake mushroom cultures.
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