Abstract
The effects of average molecular weight of PEG, concentrations of PEG and KH2PO4 and pH on the partition equilibrium of acetylspiramycin in PEG/KH2PO4 aqueous two-phase systems were studied in detail. The partition coefficients of acetylspiramycin in PEG/ KH2PO4 systems were measured at room temperature 25 °C. It was found that acetylspiramycin partitioned unevenly in the aqueous two-phase systems composed of PEG and KH2PO4 and could be purified by this technique. A suitable phase-forming system (pH=6.7, 12w/w% PEG2000, 11w/w% KH2PO4) was found out after partition coefficient (Kp=42) , extraction ratio (η=96%) and recovery ratio(R=98.8%) were investigated comprehensively in this paper.
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