Abstract
Low molecular weight peptide with a high affinity to calcium was recovered from pepsinolytic hydrolysates of Theragra chalcogramma backbone discarded from fish processing using a hydroxyapatite affinity chromatography. T. chalcogramma backbone peptide (TBP), Val-Leu-Ser-Gly-Gly-Thr-Thr-Met-Ala-Met-Tyr-Thr-Leu-Val ( M W: 1442 Da), exhibited the highest affinity to calcium ion on the surface of hydroxyapaptite (HA) crystals. In vitro calcium-binding assay elucidated that TBP can solublize a similar content of calcium with casein phosphopeptide (CPP). In the present study, it is possible to provide utilization of fish backbones in a novel nutraceutical material with a high solubility for calcium to oriental people with lactose indigestion and intolerance.
Published Version
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