Abstract
Circadian clocks provide a biochemical representation of local time inside cells and control the timing of gene expression in anticipation of sunrise and sunset. In cyanobacteria, a circadian oscillator comprised of three Kai proteins—KaiA, KaiB, and KaiC—relay temporal information downstream through two kinases, SasA and CikA, to regulate the transcription factor RpaA. Identifying the mechanisms by which circadian clocks exert temporal control over gene expression has been challenging in the complex milieu of cells.
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