Abstract
The Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo [GBCFLUX]), founded on April 24, 2010, is composed of experts in flow cytometry (FC) area who have the common objective of contributing to technical and scientific advances in Brazilian clinical and research laboratories. Among GBCFLUX working groups, the Quality Control (QC) subcommittee is responsible for discussing data in the literature and contributes to the quality assurance of the pre-analytical, analytical and post-analytical process in FC. The QC subcommittee's actions began through meetings and lectures, in which data from the literature were reviewed and discussed with all participating members of the GBCFLUX. In a second step, it was decided to draw up a text of technical and scientific consensus recommendations, informative and educative, for dissemination to all FC working groups in Brazil. To this effect, a questionnaire with objective responses was designed and sent to 35 recognized Brazilian institutions, in order to evaluate the QC profile of these institutions. Thus, the QC technical-scientific recommendations, which will be described in this updating article, are intended to ensure the process quality, technical standardization, and reproducibility of results in FC.
Highlights
Since its founding on April 24, 2010, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo [GBCFLUX]) brings together experts in order to discuss and promote technical and scientific advances in Brazilian clinical and research laboratories
Among GBCFLUX working groups, the Quality Control (QC) subcommittee is responsible for discussing data in the literature and contributes to the quality assurance of the pre-analytical, analytical and post-analytical process in flow cytometry (FC)
The GBCFLUX is composed of working committees and, among them, the flow cytometry (FC) quality control (QC) subcommittee, which is responsible for reviewing data of the literature, discussing and proposing recommendations that ensure the reliability of results and minimize potential technical failures, inherent to the method
Summary
Since its founding on April 24, 2010, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo [GBCFLUX]) brings together experts in order to discuss and promote technical and scientific advances in Brazilian clinical and research laboratories. The GBCFLUX is composed of working committees and, among them, the flow cytometry (FC) quality control (QC) subcommittee, which is responsible for reviewing data of the literature, discussing and proposing recommendations that ensure the reliability of results and minimize potential technical failures, inherent to the method. The subcommittee determined that a consensus of technical and scientific recommendations on QC should be drawn up and disclosed to all FC working groups in Brazil. To this purpose, a questionnaire with 67 objective questions was sent to 35 recognized Brazilian institutions. Collection in a dry tube is recommended; for fine-needle aspirates, lymph node tissue fragments is indicated the use of isotonic transport medium, RPMI for example.
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