Abstract
Abstract When bacteriophage S1 is grown in a Staphylococcus aureus strain carrying the nonconjugative plasmid rms7 that encodes tetracycline (TC) resistance, a phage lysate capable of transducing TC resistance at an extremely high frequency was obtained. A linear relationship was found between transduction frequency and multiplicity of phage infection and a single phage particle can form a plaque containing TC lysogenic cells in its center. Treatment with anti-S1 phage serum and heating at 63° eliminated both transducing and plaque-forming activities of the lysate. These results indicated that a single recombinant of S1 particle (called S1 ptet ) carries the tet gene(s) of the rms7 plasmid and is responsible for the transduction.
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